Identification of factors inducing catharanthine (an antileukemic drug) production in immobilised catharanthus roseus cultures

Identification of factors inducing catharanthine (an antileukemic drug) production in immobilised catharanthus roseus cultures by B.A Ingram Download PDF EPUB FB2

Catharanthus roseus is the exclusive source of an array of terpenoid indole alkaloids including the anticancer drugs vincristine and vinblastine, derived from the coupling of catharanthine and vindoline.

Leaf-synthesized vindoline is regulated by light. A seven-step enzymatic process is involved in the sequential conversion of tabersonine to vindoline; Cited by: 7. Virus-Induced Gene Silencing. Seeds of C. roseus cv. Rose Red were germinated in a greenhouse at 28°C, 16/8 h photoperiod.

Four weeks later, plants with two to three pairs of true leaves were used for VIGS. The genomic DNA of C. roseus cv.

Rose Red was used as template to amplify CR1 with specific primers (Table Table1 1).The PCR-generated fragment was then Cited by: 8. Honeydew produced by the aphid Dactynotus ambrosiae when feeding on flowering or vegetative plants of the short day plant Xanthium strumarium contains an active substance capable of inducing flowering in the long day plant Lemna gibba G3.

In the present Identification of factors inducing catharanthine book, this active material has been identified as salicylic acid through the use of gas-liquid. Vogels, M. et al. Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle.

Proteom Cited by:   Recently, several 'mesoderm-inducing factors' (MIFs) have been discovered. These cause isolated Xenopus animal caps to form mesodermal cell types such as muscle, instead of their normal fate of Cited by:   We developed a novel ultra performance liquid chromatography-quadrupole time-of-flight (UPLC-Q-TOF) mass spectrometry method that allows sensitive, rapid, and reliable detection and identification of six representative indole alkaloids (vincristine, vinblastine, ajmalicine, catharanthine, serpentine, and vindoline) that exhibit physiological activity in.

This study reported the inducing effect of Aspergillus flavus fungal elicitor on biosynthesis of terpenoid indole alkaloids (TIAs) in Catharanthus roseus cambial meristematic cells (CMCs) and its inducing mechanism.

According to the results determined by HPLC and HPLC-MS/MS, the optimal condition of the A. flavus elicitor was as follows: after suspension culture of C. roseus. An excess of NaBH4 was used for the reduction step.

Analysis of the reaction mixture indicated a maximum yield of 20% for vinblastine at pHbased on the initial amount of catharanthine concentration.

Direct-injection electrospray ionization mass spectrometry in positive ion mode was used for the identification of vinblastine. Cancer is a multistage process resulting in an uncontrolled and abrupt division of cells and is one of the leading causes of mortality. The cases reported and the predictions for the near future are unthinkable.

Food and Drug Administration data showed that 40% of the approved molecules are natural compounds or inspired by them, from which, 74% are used in anticancer therapy. In. The tetraploid plants of Catharanthus roseus (L.) G. Don was obtained by colchicine induction from seeds explants, and the ploidy of the plants was identified by flow cytometry.

The optimal treatment is % colchicine solution treated for 24 hours, and the induction rate reaches up to 30%. Comparing with morphological characteristics and growth habits between tetraploids and.

Anthony N. Rezitis, Kostas Tsiboukas, Stauros Tsoukalas, Investigation of Factors Influencing the Technical Efficiency of Agricultural Producers Participating in Farm Credit Programs: The Case of Greece, Journal of Agricultural and Applied Economics, /S, 35, 3, (), ().

Plant-Derived Anticancer Drugs: Low Supply, High Cost. Plants represent a seemingly inexhaustible source of biologically active compounds. Some of these have been essential components of the anticancer therapeutic arsenal for many years [].Unfortunately, most of these chemicals naturally accumulate in the plant in only very small quantities and their.

In this condition, the contents of vindoline, catharanthine, and ajmaline were, and times as high as those of the control group, respectively. Transcriptome analysis showed that D4H, G10H, GES, IRS, LAMT, SGD, STR, TDC, and ORCA3 were involved in the regulation of this induction process.

Plant in vitro cultures represent an attractive and cost-effective alternative to classical approaches to plant secondary metabolite (PSM) production (the “Plant Cell Factory” concept). Among other advantages, they constitute the only sustainable and eco-friendly system to obtain complex chemical structures biosynthesized by rare or endangered plant species that resist domestication.

Catharanthus roseus is an important dicotyledonous medicinal plant that produces anticancer compounds. The active alkaloids vinblastine, vindoline, ajmalicine, catharanthine, and vinleurosine were. Senescence is a tightly regulated developmental program coordinated by transcription factors.

Identifying these transcription factors in crops will provide opportunities to tailor the senescence process to different environmental conditions and regulate the balance between yield and grain nutrient content.

Here, we use ten time points of gene expression data along with gene. Suspension cultures of Catharanthus roseus (C. roseus) were elicited with fungal cell wall fragments of Aspergillus niger (), Fusarium moniliforme (F. moniliforme), and Trichoderma viride ().The effects of elicitor dosage, exposures time, and age of subculture on ajmalicine accumulation were studied.

A higher concentration of elicitor extract responded. catharanthine, and vinblast ine was ± %, ± %, and ± % on a dry weight leaves basis in the controls, respectively, while t heir average amount. Spatial separation of vindoline and catharanthine provides a clear explanation for the low levels of dimers that accumulate in intact plants.

The present work describes the molecular cloning and functional identification of a unique catharanthine transporter (CrTPT2) that is expressed predominantly in the epidermis of young leaves. View With Charts And Images Biological Investigations of Catharanthus roseus (Apocynaceae) Chapter-1 Introduction Rationale and Objective of the Present Study: Herbal medicine or botanical medicine or phytomedicine refers to the use of any plant’s seeds, berries, roots, leaves, bark, or flowers for medicinal purposes, (Barrett et al., ).

It has long (about yrs) [ ]. inducing feelings of anxiety over their own potential for decline. The social comparison process that occurs between support group members has not been thoroughly assessed Social identity theory also examines group identification with social groups.

Group identification has been found to be positively related to job.Light is indispensable to biosynthetic course of a growing plant. The key factors related light radiation include photoperiod (duration), intensity (quantity), direction and quality (frequency or wavelength) [34,35].

In the natural world, light plays an inreplaceable role in promoting plant growth and inducing or regulating plant metabolism.In book: Plant Cell Culture Protocols, pp except for the identification of an unique catharanthine transporter gene (CrTPT2) that is ex- pressed .